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51.
用奶牛乳房炎多联苗 (B)免疫家兔 ,14 d血清中金黄色葡萄球菌抗体效价可达 1∶ 8,2 8d抗体水平明显升高 ,可达 1∶ 32 ,注苗后 35 d攻毒 ,攻毒后 2 d保护率为 93.3% (14 /15 ) ,自然保护率为 2 0 % (1/5 ) ,10 d保护率为 6 6 .6 % (10 /15 ) ,自然保护率为 2 0 % (1/5 ) ;用多联苗 (B)臀部肌肉注射免疫泌乳牛 ,试验期间 4个月内 ,3ml剂量组临床型乳房炎月平均发病率为 11.0 9% ,其对照组为 2 0 .19% ,发病率降低 4 5 .2 9% ,差异极显著 (P<0 .0 1) ;5 m l剂量组月平均发病率为 13.0 1% ,其对照组为 31.36 % ,发病率降低 5 8.5 1% ,差异极显著 (P<0 .0 1) ;3ml剂量组与 5 m l剂量组之间发病率无明显差异 (P>0 .0 5 ) ;注苗后泌乳牛血清抗体水平可持续 4个月 ,且 30 d时效价水平最高。 相似文献
52.
观察34例心内直视手术体外循环前后对血液有形成份的影响,发现体外循环后血红蛋白、红细胞与血小板有显著降低,而白细胞显著增高,这可能与机械损伤、血液稀释和机体反应有关。因此选用接近人体生理循环的人工心肺机,改进手术吸引装置、缩短体外循环时间及提高手术质量,可能有助于减少体外循环对血液有形成份的影响。 相似文献
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张杰 《四川畜牧兽医学院学报》2008,(6):168-171
从1911年至1949年,中国近代美术教育是在初期的社团、师范学堂、私立、公立美术教育的基础上,形成了后期的国统区与延安鲁艺的美术教育,教育理念借鉴与效法“新式”、“兼容并包”、“通才”、“理论和实践相结合”的人才培养模式。 相似文献
57.
In China, the first Przewalski’s horse (Equus przewalskii) group was released in
Kalamaili Ungulate Protected Area in Xinjiang, in August 2001. The objective of this study
was to investigate reproduction and development of released Przewalski’s horses in
Xinjiang, China from 2002 to 2006. Twenty-four descendants were naturally born, average
reproduction rate was 38.7%, and average survival rate of foals was 69.1% in this interim.
Frequent alternation of the leading stallion and infertility in female horses due to
environmental factors were main causes for the low reproduction rate. The infant mortality
rate of released Przewalski’s horses was 25.0%, and 83.3% of death in infants was due to
the leading stallion infanticide. The released Przewalski’s horses exhibited seasonal
breeding, 70.8% of infants were born in May and June. The fertility rates were 8.3% in
April and 37.5% in May, which were lower compared to those of the captive Przewalski’s
horse groups (18.3% and 44.3%, respectively). Furthermore, the fertility rate in June was
33.3% and higher than the captive groups (18.3%). These findings showed that the breeding
peak of the released Przewalski’s horse groups was later than the captive groups, and
suggested that the altered survival environment and food supplies were the main reasons
contributing to the delayed breeding peak. 相似文献
58.
部分桂花栽培品种的AFLP分析 总被引:13,自引:0,他引:13
从22对AFLP引物中筛选出6对用来检测22个桂花品种和木犀属3个种的多态性位点,共检测到171个位点,其中多态性位点104个,占60.8%。利用DPSv 3.11软件计算25个样品之间的Nei遗传距离,并按照非加权算术平均数聚类方法(UPGMA),构建树状分支图。AFLP分析结果表明:桂花花色较深的品种之间和花色较浅的品种之间分别存在着较近的亲缘关系,而花色深浅不同的两类品种之间亲缘关系较远。从聚类图上看,22个桂花品种中最后聚在一起的分别是3个银桂品种(遗传距离0.19处)和3个四季桂品种(遗传距离0.21处),说明四季桂类和银桂类中的部分品种与金桂品种群和丹桂品种群有较远的亲缘关系。从分类上看,AFLP分析结果与传统的以形态特征为基础的分类结果并不完全一致。 相似文献
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HAN Ya-ling ZHAO Xin YAN Cheng-hui KANG Jian ZHANG Xiao-lin DENG Jie XU Hong-mei LIU Hai-wei 《园艺学报》2008,24(8):1483-1489
AIM: In order to explore the regulatory mechanisms of the human cellular repressor of E1A-stimulated genes (hCREG1) in vascular smooth muscle cells (VSMCs)-HITASY and in human umbilical vein endothelial cells (HUVECs), we clone and construct hCREG1 promoter vector to detect its expression in different vascular cells. METHODS: With the results of biological information, the fragments including -3 677 bp, -2 310 bp and -945 bp upstream sequences of hCREG1 were amplified respectively using human genomic DNA template by PCR. The products were inserted into pMD18-T vector, and then were subcloned into pEGFP-1 report vector to obtain the pEGFP-hCREG1-promoter vectors. The pEGFP-hCREG1-P3677, pEGFP-hCREG1-P2310 and pEGFP-hCREG1-P945 vectors were transfected into HITASY cells and HUVECs transiently and the expression of green fluorescent protein (GFP) was detected respectively by Western blotting and fluorescent microscope. RESULTS: It was confirmed that all three PCR fragments inserted into vectors were corrected by sequencing analysis. However, the expression of GFP was different significantly in both types of vascular cells. The expression of GFP in HUVECs was higher than that in HITASY cells. Meanwhile, the expression of GFP in HITASY cells with 0.5% FBS was increased obviously compared to that in HITASY cells with 10% FBS. CONCLUSION: The reporter vectors of hCREG1 promoter are constructed successfully in which the core promoter region might be located in -945 bp-0 bp of 5′ upstream sequences. This study will provide an experimental basis for exploring the regulation of hCREG1 expression. 相似文献